Webinar -Using Aber conductivity in CHO cell culture to detect bacterial contamination early
18th Nov.
10am EST / 3pm GMT
Dielectric spectroscopy, sometimes referred to as capacitance measurement, has been utilized for many years as a PAT tool to measure cell growth (density, in millions per mL) in bioprocesses. Apart from monitoring cell density and real time troubleshooting, capacitance technology has been used to control and automate perfusion rates, nutrient feeds, n-1 transfer, harvest point, infection point etc. It has also been used as an integral part of process development and scale up.
This webinar will explain the principle behind dielectric spectroscopy and include case studies that demonstrate its use in these applications. Apart from capacitance, dielectric spectroscopy sensors also measure conductivity. In addition to the benefits of measuring capacitance in bioprocesses, there are advantages of measuring conductivity in real time as well. Conductivity has been used as an indirect measurement of osmolarity, and thus as an indicator of the quality of the cell culture medium and for monitoring the variations in the ionic concentration of the medium during the cell culture process.
Importantly, this webinar will cover the work carried out at the US FDA, where it was demonstrated that by using in-line continuous conductivity measurements it was possible to detect bacterial contamination much earlier than by conventional means such as by the sudden decrease in dissolved oxygen (DO%) or pH, in a CHO cell culture process.
Moreover, the detection of variations in the solute concentrations in the bioreactor due to nutrient feed, bicarbonate buffer, and consequences of mammalian cellular metabolism in terms of variation in the conductivity measurements (mS/cm) could be distinguishable from the abnormal increases in conductivity corresponding to bacterial contamination, which was confirmed by orthogonal methods.
Speakers
Dr. Aditya Bhat B.Sc., M.Sc., PhD
Vice President – Technology
ABER Inc
Dr. Chikkathur Madhavarao
Office of Pharmaceutical Quality
Research in CDER, US FDA
In this webinar you will learn about
Detect bacterial
contamination early
Reliable, inline,
and real-time
PAT tool
Controlling and
automating
bioprocesses