PAT application of dielectric spectroscopy in AAV gene therapy process to improve process reproducibility and understanding


This webinar in collaboration with AskBio, gives an overview of capacitance technology, followed by examples of how the technology has been effectively applied across various applications. More specifically, we look at the PAT application of Dielectric Spectroscopy in AAV Gene Therapy processes to improve process reproducibility and understanding.


Tim Brantley

Senior Scientist, Upstream Process

Dr. Aditya Bhat

Vice President

Dielectric spectroscopy (DS), also known as capacitance measurement, has been used as a PAT tool for vaccine and viral vector production processes (adenovirus, lentivirus reovirus, influenza, baculovirus) with mammalian and insect cells. It has been viewed as an important process control tool for continuous virus production processes.

Numerous groups have explored this technology to monitor live cell density, identify critical events and control the process. The benefits of using capacitance technology in these applications are multifold. Considering capacitance measurement correlates with the live cell density (or more accurately, viable biovolume) profile of the culture, it provides a complete, real time and detailed picture of all of the critical events. This leads to better process monitoring, automation and control and in turn, improved productivity.